Development and Validation of an HPLC-MS/MS Method for Rapid Simultaneous Determination of Cefprozil Diastereomers in Human Plasma

Table 3

Stability of cis- and trans-cefprozil in human plasma under different storage conditions (n = 3).

Compound	Storage Conditions	Nominal Concentration (μg/mL)	Mean; RE (%)	CV (%)
Cis-cefprozil	Three freeze-thaw cycles	0.05	-0.7	2.4
		12.0	-4.7	0.6
	Stored at ambient temperature for 6 h	0.05	6.0	4.3
		12.0	2.1	2.4
	Stored in the auto-sampler at ambient temperature for 12h	0.05	-3.3	4.6
		12.0	0.9	6.5
	Plasma samples stored at -80°C for 30 days	0.05	-0.7	1.5
		12.0	3.3	1.1
Trans-cefprozil	Three freeze-thaw cycles	0.025	-6.7	3.2
		1.33	3.0	3.5
	Stored at ambient temperature for 6 h	0.025	5.7	5.4
		1.33	11.5	7.0
	Stored in the auto-sampler at ambient temperature for 12h	0.025	8.9	6.5
		1.33	2.1	7.0
	Plasma samples stored at -80°C for 30 days	0.025	-3.5	2.9
		1.33	10.0	6.3

CV: coefficient of variance; RE: relative error.