Comprehensive Assessment of Degradation Behavior of Simvastatin by UHPLC/MS Method, Employing Experimental Design Methodology

Figure 1

Half-normal plot and Pareto chart showing the significant effects based on the observation of Plackett–Burman design for the investigated responses (a) Rs between impurity E and F (); (b) Rs between SIM and impurity G (); (c) Rs between impurity B and C () where is molarity of ammonium formate (mM); is flow rate (mL/min); is wavelength (nm); is volume of injection (μL); is detector acquisition rate (Hz); is column temperature (°C); is percent of organic modifier in the initial mobile phase composition (%); is column of the same composition but of different lot; and is injector temperature (°C). Positive effects are marked with white bar box with orange frame and orange bar box for significant and insignificant factor, respectable. Negative effects are marked with white bar box with blue frame and blue bar box for significant and insignificant, respectable.

(a)

(b)

(c)