Comprehensive Assessment of Degradation Behavior of Simvastatin by UHPLC/MS Method, Employing Experimental Design Methodology

Table 1

Plan of Plackett–Burman design and experimentally obtained results.

Number of experiments	Factor levels									Responses
1	20	0.7	228	7	25	35	60	lot 2	25	0.72	2.50	0.92
2	20	0.3	228	3	10	35	40	lot 2	5	0.92	4.25	1.25
3	20	0.3	228	7	10	45	40	lot 1	25	1.20	3.28	6.23
4	20	0.3	240	3	25	45	60	lot 1	5	1.02	3.72	1.22
5	20	0.7	240	7	10	35	60	lot 1	5	1.03	2.02	2.21
6	20	0.7	240	3	25	45	40	lot 1	25	1.56	3.44	7.78
7	5	0.7	228	7	25	45	40	lot 2	5	1.25	3.10	1.59
8	5	0.3	228	3	25	35	60	lot 1	25	0.88	3.72	2.16
9	5	0.7	228	3	10	45	60	lot 2	5	0.71	3.56	1.26
10	5	0.3	240	7	25	35	40	lot 2	5	1.08	3.89	6.41
11	5	0.7	240	3	10	35	40	lot 2	25	1.59	4.56	4.52
12	5	0.3	240	7	10	45	60	lot 1	25	0.80	3.35	2.53

: molarity of ammonium formate (mM); : flow rate (mL/min); : wavelength (nm); : volume of injection (μL); : detector acquisition rate (Hz); : column temperature (°C); : percent of organic modifier in the initial mobile phase composition (%); : column with the same composition but different lots (lot 1: USCFZ13194/B13243; lot 2: USCFZ13193/B13243); : injector temperature (°C);: Rs E/F; : Rs G/SIM; and : Rs B/C.