Comprehensive Assessment of Degradation Behavior of Simvastatin by UHPLC/MS Method, Employing Experimental Design Methodology
Table 2
Box-Behnken experimental design matrixes of the selected independent variables and studied responses.
Number of experiments
Factors levels
Responses
1
3.8
0.6
40
3.06
1.27
1.72
2
4.2
0.6
40
2.79
1.33
1.51
3
3.8
0.8
40
3.66
1.40
1.60
4
4.2
0.8
40
4.02
1.04
1.41
5
3.8
0.7
35
3.12
1.51
1.58
6
4.2
0.7
35
4.25
1.07
1.43
7
3.8
0.7
45
3.91
1.37
1.38
8
4.2
0.7
45
2.58
1.52
1.32
9
4.0
0.6
35
3.59
1.39
1.59
10
4.0
0.8
35
3.85
1.30
1.40
11
4.0
0.6
45
3.05
1.44
1.56
12
4.0
0.8
45
3.25
1.35
1.78
13
4.0
0.7
40
4.58
1.06
2.06
14
4.0
0.7
40
4.48
1.12
2.15
15
4.0
0.7
40
4.28
1.03
2.12
: pH of mobile phase; : flow rate (mL/min); : content of acetonitrile (%); :Rs between impurity G and SIM; : tailing factor SIM (T); and :Rs between impurities B and C.
