Comprehensive Assessment of Degradation Behavior of Simvastatin by UHPLC/MS Method, Employing Experimental Design Methodology

Table 5

Obtained results from validation of the proposed method: robustness.

	Simvastatin			Res E/F	Res G/SIM	Res B/C
	Rt (min)	T	NTP
Flow (mL/min)
0.6 mL/min	14.05	0.99	109188	1.52	4.48	1.66
0.8 mL/min	13.78	1.08	128239	1.50	4.46	1.53
Content of acetonitrile in initial phase
35%	14.77	1.05	234052	1.53	4.05	1.61
45%	14.74	1.02	236896	1.58	4.17	1.66
Column temperature
30°C	13.42	1.01	93614	1.51	4.68	1.89
40°C	13.83	1.02	109994	1.53	4.43	1.78
Another column	15.07	1.05	169635	1.59	4.47	2.15