Research Article

Comparative Glycopeptide Analysis for Protein Glycosylation by Liquid Chromatography and Tandem Mass Spectrometry: Variation in Glycosylation Patterns of Site-Directed Mutagenized Glycoprotein

Figure 1

Base peak chromatogram (BPC) by LC-tandem MS analysis of the tryptic digests of reference rAb. The extracted ion chromatogram (XIC) of the glycopeptide EEQYNSTYR_3_3_1_0, identified as one of major components at RT 28.25 min, was also inserted together with the monoisotopic peak pattern of the glycopeptide.