Comparative Glycopeptide Analysis for Protein Glycosylation by Liquid Chromatography and Tandem Mass Spectrometry: Variation in Glycosylation Patterns of Site-Directed Mutagenized Glycoprotein

Figure 5

SDS-PAGE result for mutagenized rAb samples (lanes 1-3) and reference rAb glycoprotein (lane 4). Heavy chain and light chain bands were observed at 51 kDa and 26 kDa, respectively.