Research Article

Comparative Glycopeptide Analysis for Protein Glycosylation by Liquid Chromatography and Tandem Mass Spectrometry: Variation in Glycosylation Patterns of Site-Directed Mutagenized Glycoprotein

Figure 6

Comparative analysis for in-solution and in-gel rAb samples. In-solution sample is applied to a protein column and subject to tryptic digestion. In-gel sample is separated from SDS-PAGE gel and destained before tryptic digestion.