Comparative Glycopeptide Analysis for Protein Glycosylation by Liquid Chromatography and Tandem Mass Spectrometry: Variation in Glycosylation Patterns of Site-Directed Mutagenized Glycoprotein

Figure 7

Comparison of the glycosylation patterns of the reference rAb, obtained, respectively, from the in-solution and the in-gel method. The two methods for sample preparation provided comparable results, implying that the in-gel method can be complimentary to the in-solution method for mapping protein glycosylation for our purposes.