Comparative Glycopeptide Analysis for Protein Glycosylation by Liquid Chromatography and Tandem Mass Spectrometry: Variation in Glycosylation Patterns of Site-Directed Mutagenized Glycoprotein

Figure 8

Comparative glycopeptide mapping of the reference rAb and the mutagenized rAb glycoprotein by in-gel digestion and LC-ESI-MS/MS. Each dataset was normalized before comparative analysis. Glycosylation patterns were notably different between the reference rAb and the mutagenized rAb samples. Increased abundance of sialylation, galactosylation, and branching was observed in the mutagenized rAb in comparison to the reference rAb glycoprotein.