Comparative Glycopeptide Analysis for Protein Glycosylation by Liquid Chromatography and Tandem Mass Spectrometry: Variation in Glycosylation Patterns of Site-Directed Mutagenized Glycoprotein

Table 1

List of tryptic glycopeptides from triplicate analysis of reference rAb.

No.	Glycopeptides	Ion Charge (+)	Detected Mass (m/z)	Run 1		Run 2		Run 3
				RT (min)	MS_Intensity	RT (min)	MS_Intensity	RT (min)	MS_Intensity
1	EEQYNSTYR_3_3_0_0	2	1142.959	27.70	92380184	28.39	3270910	28.40	10319768
2	EEQYNSTYR_3_3_1_0	2	1215.989	27.41	372193798	28.31	72807858	28.30	188794868
		3	810.996
3	EEQYNSTYR_3_4_0_0	2	1244.499	27.70	91063963	28.39	15496872	28.42	15407082
		3	830.000
4	EEQYNSTYR_3_4_1_0	2	1318.029	27.40	1541540913	28.28	398363214	28.30	815484054
		3	878.688
5	EEQYNSTYR_3_5_1_0	3	946.380	27.60	7447300	28.32	5456578	28.30	7199469
6	EEQYNSTYR_4_2_0_0	2	1122.443	27.60	126933115	28.39	1113753	28.35	6721632
7	EEQYNSTYR_4_3_0_1	3	913.355	28.28	188888	28.88	2653427	28.90	1300713
8	EEQYNSTYR_4_3_1_0	2	1297.018	27.40	50607743	28.25	6312278	28.21	20746171
9	EEQYNSTYR_4_3_1_1	2	1442.557	28.10	29760350	28.81	55607576	28.80	28356277
		3	962.043
10	EEQYNSTYR_4_4_1_0	2	1398.554	27.38	285767417	28.25	58136525	28.20	205614717
		3	932.706
11	EEQYNSTYR_4_4_1_1	3	1029.738	28.10	5127014	28.80	36633653	28.85	12664730
12	EEQYNSTYR_4_5_1_0	3	1000.398	27.54	2426554	28.32	4640770	28.30	6085787
13	EEQYNSTYR_5_2_0_0	2	1203.473	27.50	542316083	28.28	16939021	28.29	69462798
		3	802.652
14	EEQYNSTYR_5_4_1_0	3	986.723	27.36	3496656	28.10	2860860	28.10	10937706
15	EEQYNSTYR_5_4_1_1	3	1083.755	28.10	8174096	28.81	18861846	28.80	25603836
16	EEQYNSTYR_5_4_1_2	3	1180.784	28.52	1129042	29.20	3397473	29.29	1181060
17	EEQYNSTYR_6_2_0_0	2	1284.497	27.40	102132925	28.18	733668	28.21	14024518
18	EEQYNSTYR_6_3_1_0	3	973.049	27.36	4256849	28.18	638835	28.20	4721699
19	EEQYNSTYR_6_3_1_1	3	1070.081	28.04	5703122	28.74	18933930	28.70	8336957
20	EEQYNSTYR_7_2_0_0	2	1365.524	27.40	55356342	28.18	559700	28.21	5262829

MS_Intensity is based on the summation of 3-top monoisotopic mass peak intensity [21]. If simultaneously detected, MS_Intensity values of 2⁺ and 3⁺ mass ions are summed.