International Journal of Analytical Chemistry

Research Article

Effects of Different Methods of Isolation on Volatile Composition of Artemisia annua L.

Table 1

Volatile constituents of Artemisia annua L. obtained by different methods of isolation.


Compound	RI^a	RI^b	Sample ()
			A	B	C	D	E

Ethyl 2-methyl butanoate	869		0.3	-	-	-	-
Santolina triene	910		0.5	-	-	-	-
Artemisia triene	929		-	0.4	0.2	-	-
α-Pinene	937	1043	0.3	t	-	11.9	-
Camphene	951	1061	6.5	0.8	0.6	25.6	-
Sabinene	972	1117	0.5	0.2	t	6.2	-
β-Pinene	976	1102	t	t	-	t	-
β-Myrcene	984	1145	17.1	3.7	4.2	1.7	0.9
Yomogi alcohol	997	1370	-	0.5	3.9	-	-
p-Cymene	1025	1244	-	t	t	-	-
1,8-Cineole	1031	1193	8.5	5.3	5.4	18.3	0.3
Artemisia ketone	1065	1330	46.4	30.2	28.3	24.5	16.5
cis-Sabinene hydrate	1076	1524	-	t	-	-	-
Artemisia alcohol	1087	1475	2.5	3.1	2.2	0.5	2.8
trans-Sabinene hydrate	1100	1423	-	0.8	0.4	-	0.7
trans-Pinocarveol	1141	1603	-	0.5	0.4	-	t
Camphor	1151	1476	13.2	24.0	16.9	7.5	20.0
Pinocarvone	1167		-	0.7	0.4	-	-
Borneol	1172	1657	-	t	t	-	-
Terpinen-4-ol	1175	1560	-	0.7	0.7	-	1.5
p-Cymen-8-ol	1181		-	t	t	-	t
α-Terpineol	1187	1650	-	1.3	0.7	-	0.8
Myrtenal	1209	1580	-	0.7	0.4	-	-
Myrtenol	1210	1730	-	1.0	0.4	-	-
cis-3-Hexenyl 2-methylbutanoate	1217		0.1	-	-	-	-
Hexyl 2-methylbutanoate	1219		-	0.6	0.4	-	0.5
3-Tetradecene	1274		0.2	0.2	0.2	-	-
p-Cymen-7-ol	1280		-	t	t	-	-
Piperitenone	1342	1856	-	1.1	0.8	-	-
Eugenol	1358	2091	-	0.9	1.2	-	-
α-Copaene	1384	1466	-	0.7	0.7	-	0.5
Phenyl methyl pentanoate	1387	1813	-	0.1	0.4	-	0.5
β -Cubebene	1390	1666	-	1.7	0.8	-	-
β-Elemene	1396		-	0.8	0.6	-	-
β-Caryophyllene	1426	1565	0.1	3.1	3.9	1.3	1.2
α-Humulene	1456	1620	t	t	0.6	-	-
(E)-β-Farnesene	1458	1634	0.2	4.2	4.1	0.4	t
τ-Selinene	1476	1646	0.1	1.8	1.3	-	-
γ-Muurolene	1478		0.1	t	t	-	-
Germacrene -D	1493	1689	-	0.6	0.6	-	-
β-Selinene	1495	1675	t	0.4	0.3	-	1.0
Viridiflorene	1497	1663	t	t	t	-	-
Isobornyl-3-methylbutanoate	1513		-	t	-	-	0.5
Caryophyllene oxide	1590	1930	-	2.4	2.6	-	8.2
1-epi-Cubenol	1619	2006	-	0.7	0.8	-	1.8
Selina-3,11-diene-6-α-ol	1631		-	0.8	0.6	-	2.7
Selin-11-en-4-α-ol	1649		-	0.5	0.4	-	1.9
α-Bisabolol	1664	2164	-	0.4	0.5	-	1.4
Hexahydrofarnesyl acetone	1779		-	0.4	2.4	-	0.7
Phytol	1970		-	0.6	-	-	-

Monoterpenes hydrocarbons (MH)			24.9	5.1	5.0	45.4	0.9
Oxygenated monoterpenes (MO)			70.6	69.9	60.9	50.8	42.6
Sesquiterpene hydrocarbons (SH)			0.5	13.3	13.0	1.7	2.6
Oxygenated sesquiterpenes (SO)			-	4.9	4.9	-	15.9
Oxygenated diterpenes (DO)			0.6	2.2	4.6	-	2.3
Others (O)			-	0.6	-	-	-

Total			96.5	96.1	88.3	97.8	64.3

, retention indices relative to C₈–C₂₄, n-alkanes on the PE-5 capillary column; , retention indices relative to C₈–C₂₄, n-alkanes HP-20M capillary column; sample A: headspace of plant material of A. annua; sample B: essential oil isolated by steam distillation; Sample C: essential oil isolated by hydrodistillation; sample D: headspace of petroleum ether extract; sample E: steam distillation of petroleum ether extract; %: relative percentage obtained from peak area; t<0.1%. Percentages of dominant compounds are given in bold.