An LC-MS/MS Method for Simultaneous Determination of the Toxic and Active Components of Cortex Periplocae in Rat Plasma and Application to a Pharmacokinetic Study

Table 1

The accuracy, precision, recoveries, and matrix effects.

Compounds	Concentration (ng/mL)	Intra-day		inter-day		Recovery		Matrix effect
		Accuracy (%)	RSD (%)	Accuracy (%)	RSD (%)	Accuracy (%)	RSD (%)	Normalized matrix factor	RSD (%)
isovanillin	7.2	106	3.58	99.6	5.18	97.1	3.56	1.08	4.45
	120	111	2.19	102	13.0	97.1	2.31	1.06	2.46
	1200	108	2.55	105	7.59	104	1.73	1.04	1.79
scopoletin	1.44	108	2.92	107	1.88	102	6.68	1.00	3.32
	24	109	2.77	106	2.13	103	5.39	0.98	1.91
	240	96.4	4.51	95.2	2.33	98.1	2.81	1.06	2.49
periplocin	7.2	111	4.26	115	3.82	100	7.79	1.01	10.7
	120	109	4.02	104	3.21	97.7	6.67	0.98	4.18
	1200	101	6.34	110	6.07	106	3.40	0.99	2.06
periplogenin	1.44	106	3.69	108	1.32	90.9	5.60	0.98	9.71
	24	110	2.71	107	4.21	98.0	6.23	0.97	1.68
	240	95.4	4.03	97.9	4.11	102	2.19	0.91	3.18
periplocymarin	1.44	101	7.64	107	8.09	103	9.75	0.87	7.79
	24	106	3.70	102	7.25	99.5	5.94	0.96	3.96
	240	101	3.20	95.3	8.76	104	1.97	0.89	1.96
IS1	300	-	-	-	-	81.6	2.12	-	-
IS2	20	-	-	-	-	88.6	6.18	-	-