Optimization of Resolving Power, Fragmentation, and Mass Calibration in an Orbitrap Spectrometer for Analysis of 24 Pesticide Metabolites in Urine

Figure 1

CID fragmentation of PBA (m/z = 169.06589). Variation of the fragment ion response (peak area) with the applied energy (eV). (1) Applying only ESI⁺; (2) using the polarity switching (ESI⁺, ESI^- in the same injection). Acquisition conditions: R= 25,000 FWHM and external mass calibration.