Simultaneous Determination of Schisandrin and Promethazine with Its Metabolite in Rat Plasma by HPLC-MS/MS and Its Application to a Pharmacokinetic Study

Table 4

Recovery and matrix effect of the three analytes in rat plasma.

Compound	Nominal concentration (ng/mL)	Recovery (%)			Matrix effect (%)
		Recovery	SD	Bias (%)	Matrix	SD	Bias (%)
Promethazine	1.25	89.81	5.04	5.62	104.36	6.35	6.08
	12.5	87.17	2.44	2.80	103.74	3.24	3.13
	150	84.50	5.26	6.23	102.37	1.57	1.53
Promethazine sulfoxide	1.25	99.24	4.84	4.88	121.56	8.10	6.67
	12.5	96.70	4.74	4.90	122.80	16.34	13.31
	150	100.44	3.19	3.18	122.06	10.09	8.27
Schisandrin	1.25	97.92	5.07	5.17	105.19	11.70	11.12
	12.5	99.79	10.43	10.45	109.68	14.98	13.66
	150	98.65	4.31	4.37	106.51	10.63	9.98