Simultaneous Determination of Seven Active Components in Rat Plasma by UHPLC-MS/MS and Application to a Quantitative Study after Oral Administration of Huang-Lian Jie-Du Decoction in High Fat-Induced Atherosclerosis Rats

Table 3

Intra-/inter-day precision and accuracy for the determination of the components in rat plasma.

Analytes	Spiked Concentration; (ng/mL)	Precision (%)		Accuracy (%, Mean ± SD)
		Intra-day	Inter-day	Intra-day	Inter-day
Baicalin	2.1	6.54	2.96	85.12 ± 5.82	90.10 ± 9.84
	25.0	5.82	10.87	90.73 ± 7.80	87.45 ± 4.95
	2625	10.76	11.71	91.19 ± 8.37	94.14 ± 7.30
Baicalein	2.0	6.60	5.41	95.19 ± 8.95	92.61 ± 7.28
	25.0	11.72	7.98	96.46 ± 8.17	98.19 ± 6.89
	575	6.86	4.24	100.15 ± 9.36	105.85 ± 7.94
Wogonoside	2.0	3.45	14.31	87.12 ± 5.84	102.19 ± 9.78
	25.0	7.85	7.54	90.73 ± 7.91	85.96 ± 5.85
	1875	7.19	0.77	101.14 ± 5.38	98.89 ± 7.23
Wogonin	2.0	2.91	5.97	96.45 ± 5.89	88.77 ± 4.93
	25.7	4.53	14.11	92.76 ± 6.89	94.75 ± 5.73
	136.7	2.06	9.92	95.71 ± 8.78	90.50 ± 3.35
Berberine	0.25	3.92	13.28	88.19 ± 6.59	88.14 ± 5.05
	2.50	9.48	2.99	99.45 ± 8.84	85.79 ± 8.78
	25.0	1.29	12.25	100.89 ± 8.73	91.16 ± 4.38
Palmatine	0.25	3.75	10.54	85.12 ± 5.81	95.19 ± 8.95
	2.50	7.36	9.66	90.89 ± 7.93	106.49 ± 8.19
	25.0	6.28	0.78	108.78 ± 4.73	110.14 ± 9.24
Jatrorrhizine	0.25	3.55	13.06	104.12 ± 7.46	87.02 ± 6.24
	2.50	7.18	6.57	92.57 ± 6.65	90.64 ± 5.93
	25.0	4.38	0.86	98.10 ± 4.35	96.18 ± 4.97