Simultaneous Determination of Seven Active Components in Rat Plasma by UHPLC-MS/MS and Application to a Quantitative Study after Oral Administration of Huang-Lian Jie-Du Decoction in High Fat-Induced Atherosclerosis Rats

Table 5

Stability of the components in rat plasma under a variety of storage and process conditions.

Analyte	Spiked Concentration; (ng/mL)	RSD%
		Freeze-thaw cycles	Short-term stability	Long-term stability	Auto-sampler stability
		(three freeze-thaw cycles)	(room temperature, 4 h)	(-20°C, 30 d)	(4°C, 12 h)
Baicalin	2.1	13.86	10.53	5.63	6.93
	25.0	5.34	4.56	5.91	8.72
	2625	10.64	8.45	1.25	2.16
Baicalein	2.0	8.17	6.48	3.99	12.79
	25.0	7.89	5.78	6.23	7.89
	575	3.73	6.78	5.69	11.36
Wogonoside	2.0	5.54	3.91	2.46	7.88
	25.0	10.35	4.99	14.16	2.52
	1875	11.95	11.56	11.99	8.38
Wogonin	2.0	4.25	8.72	1.96	4.53
	25.7	7.11	12.12	3.79	3.73
	136.7	13.62	10.56	2.88	4.79
Berberine	0.25	12.30	7.63	3.73	7.82
	2.50	11.01	8.43	12.19	10.13
	25.0	14.52	4.96	13.13	7.40
Palmatine	0.25	15.75	8.45	13.83	9.93
	2.50	11.82	8.31	11.98	6.67
	25.0	10.07	4.97	2.54	7.99
Jatrorrhizine	0.25	11.20	7.54	2.86	7.87
	2.50	11.16	6.08	2.11	11.69
	25.0	14.82	9.05	1.31	4.09