Table 5: Stability of the components in rat plasma under a variety of storage and process conditions.

AnalyteSpiked Concentration
(ng/mL)
RSD%
Freeze-thaw cyclesShort-term stabilityLong-term stabilityAuto-sampler stability
(three freeze-thaw cycles)(room temperature, 4 h)(-20°C, 30 d)(4°C, 12 h)

Baicalin2.113.8610.535.636.93
25.05.344.565.918.72
262510.648.451.252.16
Baicalein2.08.176.483.9912.79
25.07.895.786.237.89
5753.736.785.6911.36
Wogonoside2.05.543.912.467.88
25.010.354.9914.162.52
187511.9511.5611.998.38
Wogonin2.04.258.721.964.53
25.77.1112.123.793.73
136.713.6210.562.884.79
Berberine0.2512.307.633.737.82
2.5011.018.4312.1910.13
25.014.524.9613.137.40
Palmatine0.2515.758.4513.839.93
2.5011.828.3111.986.67
25.010.074.972.547.99
Jatrorrhizine0.2511.207.542.867.87
2.5011.166.082.1111.69
25.014.829.051.314.09