Application of Multivariate Statistical Analysis to Simultaneous Spectrophotometric Enzymatic Determination of Glucose and Cholesterol in Serum Samples

Table 1

Parameters of PLS model (400-600 nm, 5 nm interval).

	Analytes	Calibration		Cross-validation
Model		R^2	RMSEC (mg/dL)	R^2	RMSECV (mg/dL)	PRESS
PLS	Glucose	0.9469	0.0376	0.5931	0.1041	0.9764
	Cholesterol	0.9222	0.0455	0.1594	0.1497	2.0174
PLS, smoothing 5 pts	Glucose	0.9483	0.0383	0.5633	0.1076	1.0499
	Cholesterol	0.9252	0.0446	0.3739	0.1292	1.5025