A New Quinone Based Fluorescent Probe for High Sensitive and Selective Detection of Biothiols and Its Application in Living Cell Imaging

Figure 9

Confocal laser scanning microscopy images of living Eca9706 cells. (a) Fluorescence image of Eca9706 cells incubated with 0.15 mg/ml DDQ for 40 min at 37°C. (b) Bright-field transmission image of Eca9706 cells shown in panel (1). (c) Overlay image of (a) and (b). (d) Fluorescence image of Eca9706 cells pretreated with 1mM N-methylmaleimide (a masking reagent for thiols) at 37°C for 30 min and then cultured with 0.15 mg/ml DDQ for 40 min at 37°C. (e) Bright-field transmission image of Eca9706 cells shown in panel (d). (f) Overlay image of (d) and (e).

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