Optimization of Flavonoid Extraction in<i> Dendrobium officinale</i> Leaves and Their Inhibitory Effects on Tyrosinase Activity

<table class="fixed-width table-group" id="tab4"><tr><td><table class="table"><colgroup><col style="width:8.87em"/><col style="width:2.61em"/><col style="width:2.56em"/><col style="width:4.00em"/><col style="width:3.56em"/></colgroup><tr><td class="thead-hr" colspan="5"><hr/></td></tr><tr class="thead"><td class="align_left">Reagent /Number</td><td class="align_center">A<sub>0</sub></td><td class="align_center">A<sub>1</sub></td><td class="align_center"><span style="width: 36.6422ptpx;"><svg height="14.067pt" id="M4" style="vertical-align:-5.3006pt" version="1.1" viewbox="-0.0498162 -8.7664 36.6422 14.067" width="36.6422pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M673 0V28C608 34 594 43 563 129C499 303 432 494 370 665L339 656L132 132C98 43 84 37 20 28V0H238V28C162 35 154 45 175 108C188 150 203 192 219 237H432C454 176 474 122 487 83S491 36 428 28V0H673ZM418 280H234C265 362 296 450 328 535H330L418 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303 432 494 370 665L339 656L132 132C98 43 84 37 20 28V0H238V28C162 35 154 45 175 108C188 150 203 192 219 237H432C454 176 474 122 487 83S491 36 428 28V0H673ZM418 280H234C265 362 296 450 328 535H330L418 280Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,8.983,3.132)"><path d="M156 406V710C117 695 54 682 9 675V647C73 642 75 635 75 573V25C131 -2 180 -12 223 -12C359 -12 478 92 478 240C478 357 387 451 278 451C265 451 251 447 236 440L156 406ZM156 373C175 384 208 392 235 392C315 392 387 328 387 214C387 97 332 30 251 31C197 31 170 66 162 84C158 93 156 104 156 118V373Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,13.624,3.132)"><path d="M244 0V30C178 35 170 41 170 107V710C136 697 72 680 18 675V647C83 642 89 639 89 572V107C89 43 80 35 16 30V0H244Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,15.99,3.132)"><path d="M442 39L431 68C420 63 406 57 394 57C377 57 359 71 359 118V301C359 353 349 393 314 424C291 443 261 451 230 451C171 438 107 401 79 381C59 366 46 355 46 340C46 316 72 295 91 295C106 295 117 303 122 319C131 350 139 371 152 384C163 397 178 404 197 404C246 404 281 365 281 293V278C260 262 186 232 124 213C69 194 41 161 41 112C41 47 92 -12 163 -12C195 -12 243 25 283 51C288 35 293 22 308 7C319 -4 339 -12 354 -12L442 39ZM281 87C262 68 226 51 199 51C169 51 129 74 129 126C129 162 151 181 190 198C211 208 259 229 281 240V87Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,19.993,3.132)"><path d="M532 0V30C473 38 467 38 467 107V298C467 395 416 451 336 451C307 451 279 439 251 420C226 404 205 389 185 374V453C142 434 94 422 43 413V386C98 378 104 373 104 309V107C104 41 100 38 31 30V0H251V30C191 35 185 40 185 107V337C216 364 255 389 295 389C360 389 386 347 386 276V110C386 44 380 35 321 30V0H532Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,25.108,3.132)"><path d="M523 0V30C483 33 455 48 422 83C390 115 285 234 266 263C309 314 327 333 351 357C388 392 414 404 483 415V444L273 435V405C320 400 321 392 302 364C285 339 256 300 233 277C218 262 201 250 170 243V710C130 697 73 680 22 675V647C85 642 89 637 89 570V107C89 44 83 35 14 30V0H244V30C179 35 170 41 170 107V214C176 216 181 217 186 217C194 217 203 212 216 196C273 129 333 54 384 -1L523 0Z"></path></g></svg></span></td></tr><tr><td class="thead-hr" colspan="5"><hr/></td></tr><tr><td class="align_left">L-tyrosine /L-dopa</td><td class="align_center">100</td><td class="align_center">0</td><td class="align_center">100</td><td class="align_center">0</td></tr><tr><td class="align_left">Tyrosinase</td><td class="align_center">30</td><td class="align_center">30</td><td class="align_center">30</td><td class="align_center">30</td></tr><tr><td class="align_left">Inhibitor</td><td class="align_center">0</td><td class="align_center">0</td><td class="align_center">10</td><td class="align_center">10</td></tr><tr><td class="align_left">Buffer</td><td class="align_center">70</td><td class="align_center">170</td><td class="align_center">60</td><td class="align_center">160</td></tr><tr class="table-tr"><td colspan="5"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

International Journal of Analytical Chemistry

tab4

Table 4

Table 4: Optimization of Flavonoid Extraction in<i> Dendrobium officinale</i> Leaves and Their Inhibitory Effects on Tyrosinase Activity 

International Journal of Analytical Chemistry

Optimization of Flavonoid Extraction in Dendrobium officinale Leaves and Their Inhibitory Effects on Tyrosinase Activity

Table 4