Research Article
Application of Factorial and Doehlert Designs for the Optimization of the Simultaneous Separation and Determination of Antimigraine Drugs in Pharmaceutical Formulations by RP-HPLC-UV
Figure 5
Typical chromatograms of real samples: Zomig tablets, Naramig tablets, Ikaran LP tablets, Pizofen tablets, and Pizofen syrup. Mobile phase: 38 : 62 (v/v) ACN : sodium perchlorate buffer (10 mM) at pH 3.5; column: Waters XBridge C18 (250 mm × 4.6 mm, 5 μm); detection at 220 nm; flow rate: 1.0 mL·min−1. Identification: 1 = maleic acid, 2 = zolmitriptan, 3 = naratriptan, 4 = ketotifen, 5 = dihydroergotamine, 6 = pizotifen, 7 = methylparaben, and 8 = propylparaben.