Analysis of Acrylamide in Dried Blood Spots of Lung Cancer Patients by Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry

Table 2

Summary of the full validation result.

Analyte	QC	Conc. (μg/mL)	Precision (%CV)		Accuracy (%bias)		Matrix effect		Internal standard-normalized matrix factor
			Within run	Between run	Within run	Between run	Mean % ± SD	%CV	Mean % ± SD	%CV
AA	LLOQ	2.50	7.07	9.51	−10.35	−11.73
	QCL	7.50	3.89	4.12	6.12	9.88	105.68 ± 12.34	11.68	0.91 ± 0.13	14.11
	QCM	50.0	3.12	4.48	9.49	13.71
	QCH	75.00	1.89	5.41	−6.29	11.75	96.33 ± 8.77	9.11	0.98 ± 0.10	10.11
Propranolol							117.05 ± 5.77	4.93

QC: quality control, LLOQ: lower limit of quantification, QCL: low level, QCM: medium level, QCH: high level, and CV: variability, number of experiments.