Development of the High Sensitivity and Selectivity Method for the Determination of Histamine in Fish and Fish Sauce from Vietnam by UPLC-MS/MS

Table 5

Recovery, intraday repeatability (day 1), and interday repeatability assay (day 3, day 5, and day 7) measured as %RSD of spiked fish sauce and fish samples (1.0 mL of fish sauce or 1.0 g of the fish sample, 50 mL of sample volume, and 20 times of dilution factor).

Samples	Concentration of histamine in the sample (ng/mL)	Spiked concentration (mg/L)	Recovery, R (%)	Repeatability (intraday), RSD (%)
				Day 1	Day 3	Day 5	Day 7
Fish sauce, TFS 18	385.6	214	109.21	1.83	2.34	3.51	6.71
		428	100.86	2.55	3.22	8.34	4.29
		856	116.43	1.81	2.44	6.65	10.21
Mackerel fish	64.5	25	105.32	2.35	3.12	3.59	6.71
		50	95.14	3.49	3.67	6.15	6.91
		100	107.21	2.92	3.13	7.14	11.52