International Journal of Analytical Chemistry / 2020 / Article / Tab 3

Research Article

Development of an Enzyme-Linked Immunosorbent Assay Method for the Detection of Rhein in Rheum officinale

Table 3

Recovery of rhein determined by icELISA in spiked samples.

Spiked level (μg)Mean amounta (μg)Recoveryb (%)

03.17 ± 0.29c0
14.06 ± 0.0589.17 ± 4.56
25.38 ± 0.23102.90 ± 6.96
57.38 ± 0.2984.19 ± 5.72
1013.15 ± 0.1099.77 ± 1.00

aData were mean ± SD from triplicate samples for each level. bThe percentage of recovery was calculated as follows: recovery (%) = (measured amount − control)/spiked amount × 100. cThe zero spiked level was obtained by the HPLC method and used as a control.

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