Research Article

Development of an Enzyme-Linked Immunosorbent Assay Method for the Detection of Rhein in Rheum officinale

Table 4

Determination of rhein contents in Rheum officinale samples by icELISA and HPLC.

SamplesaConcentrations of rhein (mg g−1)
icELISAHPLC

Gansu3.99 ± 0.20b3.59 ± 0.08
Anguo, Hebei3.66 ± 0.223.52 ± 0.01
Bozhou, Anhui3.33 ± 0.193.15 ± 0.09
Bozhou, Anhui3.48 ± 0.293.20 ± 0.03
Chengdu, Sichuan4.30 ± 0.294.14 ± 0.02
Chengdu, Sichuan3.60 ± 0.353.26 ± 0.01
Ganzhi, Sichuan4.59 ± 0.364.23 ± 0.04

aEach sample was analyzed in triplicate. bThe data represented the mean ± SD.