Development and Validation of UPLC-MS/MS Method for Determination of Enasidenib in Rat Plasma and Its Pharmacokinetic Application

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="6"><hr/></td></tr><tr class="thead"><td class="align_left" rowspan="2">Analyte</td><td class="align_center" rowspan="2">Concentration added (ng/mL)</td><td class="align_center" colspan="2">Recovery (%)</td><td class="align_center" colspan="2">Matrix effect (%)</td></tr><tr class="thead"><td class="align_center">Mean ± SD</td><td class="align_center">RSD (%)</td><td class="align_center">Mean ± SD</td><td class="align_center">RSD (%)</td></tr><tr><td class="thead-hr" colspan="6"><hr/></td></tr><tr><td class="align_left" rowspan="3">Enasidenib</td><td class="align_center">2.5</td><td class="align_center">80.07 ± 2.44</td><td class="align_center">11.4</td><td class="align_center">99.09 ± 13.88</td><td class="align_center">14.01</td></tr><tr><td class="align_center">400</td><td class="align_center">82.74 ± 2.36</td><td class="align_center">3.1</td><td class="align_center">99.35 ± 5.75</td><td class="align_center">5.79</td></tr><tr><td class="align_center">800</td><td class="align_center">84.83 ± 1.44</td><td class="align_center">1.8</td><td class="align_center">100.04 ± 1.98</td><td class="align_center">1.98</td></tr><tr class="table-tr"><td colspan="6"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Recovery and matrix effect of enasidenib in rat plasma (<i>n</i> = 6).</div>

International Journal of Analytical Chemistry

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Table 2

Table 2: Development and Validation of UPLC-MS/MS Method for Determination of Enasidenib in Rat Plasma and Its Pharmacokinetic Application 