A Simple Spectrophotometric Method for Determination of Glyoxylic Acid in Its Synthesis Mixture

Table 1

Comparison between spectrophotometric and HPLC methods in terms of standard deviation, recovery%, and RSD%.

Method	Glyoxylic acid concentration in the mixture M	Determined glyoxylic acid in the mixture M	Standard deviation ± M	Recovery (%)	RSD (%)
Spectrophotometric	0.35	0.354	0.00132	101	0.37
	0.50	0.504	0.00110	109	0.22
HPLC	0.35	0.361	0.00466	103	1.29
	0.50	0.508	0.00457	102	0.90

Samples diluted 25 times in the spectrophotometric determination only. RSD: relative standard deviation.