Quantification of Soluble or Insoluble Fractions of Leishmania Parasite Proteins in Microvolume Applications: A Simplification to Standard Lowry Assay

Table 1

Assessment of precision/accuracy of the assay.

	Absorbance values of different BSA (μg/ml)
Replicates	10	30	100	150	300	500
Mean (M)	0.150	0.182	0.228	0.271	0.423	0.622
Standard deviation (SD)	0.006	0.006	0.011	0.015	0.028	0.044
2SD	0.012	0.013	0.022	0.030	0.056	0.088
Upper limit (M + 2SD)	0.162	0.195	0.249	0.301	0.479	0.710
Lower limit (M − 2SD)	0.138	0.170	0.206	0.241	0.367	0.534
Coefficient of variation (CV)	3.970	3.495	4.828	5.451	6.613	7.077