Research Article
Quantification of Soluble or Insoluble Fractions of Leishmania Parasite Proteins in Microvolume Applications: A Simplification to Standard Lowry Assay
Table 2
Sensitivity ranges of most established protein assay methods.
| Protein assay method | Sensitivity range |
| Biuret method [6] | 1–10 mg | Hartree’s modified Lowry assay [7] | 15–110 μg | Bradford or Coomassie brilliant blue method [13] | 1–200 μg | Colloidal gold method [18] | 20–640 ng | Bicinchoninic acid assay (Smith or BCA method) [15] | 0.2–50 μg | Lowry protein assay [11] | 2–100 μg | The proposed method | 1–50 μg |
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