Quantification of Soluble or Insoluble Fractions of Leishmania Parasite Proteins in Microvolume Applications: A Simplification to Standard Lowry Assay

Table 3

Assessment of the precision of the antigen coating procedure using three different coating buffers.

Replicates	PBS (1X, pH 7.4)	Phosphate buffer (0.02 M, pH 7.8)	Carbonate buffer (0.05 M, pH 9.6)
1	0.022	0.030	0.023
2	0.027	0.081	0.018
3	0.028	0.028	0.023
4	0.026	0.029	0.022
5	0.021	0.020	0.023
6	0.023	0.030	0.019
7	0.022	0.057	0.018
8	0.023	0.046	0.021
9	0.030	0.021	0.021
10	0.026	0.020	0.019
M	0.025	0.036	0.021
SD	0.003	0.020	0.002
CV	12.018	54.379	9.940
Ag, μg/ml	12.981	20.125	10.438
Mean amount of coated antigen (μg)	1.298	2.013	1.044