Quantification of Soluble or Insoluble Fractions of Leishmania Parasite Proteins in Microvolume Applications: A Simplification to Standard Lowry Assay

Table 4

Comparison of reporting method with other commercially available Lowry assay kits.

Feature	Proposed method	Some commercially available Lowry assay kits (brand specifications are not provided)
		Assay A	Assay B	Assay C
Linear quantification range	10–500 μg/ml	Nonlinear	0–10 μg/ml	5–100 μg/ml
Use of micro-well plate methods	Yes	Yes	Yes	Yes
Total reaction volume per well	240 μl	260 μl	320 μl	130 μl
Total incubation time	55 min	41 min	45 min	40 min