Establishment and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Tigecycline in Critically Ill Patients

Table 1

The precision and accuracy of the determination of TGC in human plasma (n = 6).

Nominal concentration (ng/ml)	Intrabatch (n = 6)			Interbatch (n = 18)
	Measured concentration (ng/ml, mean ± SD)	CV%	Accuracy%	Measured concentration (ng/ml, mean ± SD)	CV%	Accuracy%
50	49 ± 1.8	3.6	98.6	47 ± 3.3	7.2	93.4
150	153 ± 2.7	1.8	101.8	150 ± 5.5	3.7	99.7
750	730 ± 12.7	1.7	97.3	716 ± 30.2	4.2	95.4
2250	2277 ± 48.0	2.1	101.3	2206 ± 112.8	5.1	98.1
3800	3565 ± 105.4	3.0	93.8	3739 ± 214.2	5.7	98.4