Establishment and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Tigecycline in Critically Ill Patients

Table 3

Stability of TGC in human plasma under tested conditions.

Stability	Nominal concentration (ng/mL)	Stability sample concentration (ng/mL)	Accuracy (%)	CV (%)
Short‐term (24 h, 25°C)	150	168 ± 7.0	112.3	4.2
	3800	3753 ± 206.5	98.8	5.5
Short‐term (24 h, 4°C)	150	158 ± 14.7	105.4	9.3
	3800	3673 ± 212.0	96.8	5.8
Freeze-thaw (five cycles, −80−25°C)	150	150 ± 3.8	100.1	2.5
	3800	3993 ± 293.6	105.1	7.4
Long‐term (130 days, −80°C)	150	156 ± 11.1	103.8	7.1
	3800	3524 ± 97.4	92.7	2.8
Autosampler (30 h, 30°C)	150	162 ± 10.4	108.2	6.4
	3800	4021 ± 269.0	105.8	6.7