Development and Validation of a Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry Method for the Determination of Asparagine in Human Serum

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left">Nominal conc. (<i>μ</i>M)</td><td class="align_center">Matrix effect (%)</td><td class="align_center">IS-normalized matrix effect</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left">6</td><td class="align_center">63.49 ± 4.94</td><td class="align_center">100.91 ± 13.19</td></tr><tr><td class="align_left">80</td><td class="align_center">63.34 ± 1.16</td><td class="align_center">94.64 ± 4.73</td></tr><tr><td class="align_left">150</td><td class="align_center">65.83 ± 0.53</td><td class="align_center">96.10 ± 2.09</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Matrix effect for the determination of ASN (<i>n</i> = 4).</div>

International Journal of Analytical Chemistry

tab1

Table 1

Table 1: Development and Validation of a Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry Method for the Determination of Asparagine in Human Serum 

Table 1 | Development and Validation of a Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry Method for the Determination of Asparagine in Human Serum 