Development and Validation of a Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry Method for the Determination of Asparagine in Human Serum

Table 2

Precision and accuracy for the determination of ASN (n = 6).

Nominal conc. (μM)	Backcalculated conc. (μM)	Accuracy (%)	Precision (CV, %)
			Intrabatch	Interbatch
2	1.99 ± 0.27	99.44 ± 13.52	15.02	14.74
6	5.51 ± 0.45	91.80 ± 7.48	8.54	11.21
80	79.94 ± 4.15	99.93 ± 5.18	5.96	3.89
150	145.24 ± 6.83	96.82 ± 4.55	5.03	6.02