Development and Validation of a Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry Method for the Determination of Asparagine in Human Serum

<table class="table-group" id="tab3"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left" rowspan="2">Storage condition</td><td class="align_center" colspan="2">Recovery (%)</td></tr><tr class="thead"><td class="align_center">LQC (6 <i>μ</i>M)</td><td class="align_center">HQC (150 <i>μ</i>M)</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left">Benchtop stability (48 h)</td><td class="align_center">101.89 ± 0.59</td><td class="align_center">102.87 ± 0.98</td></tr><tr><td class="align_left">Long-term stability (−70°C, 87 days)</td><td class="align_center">96.83 ± 1.48</td><td class="align_center">99.90 ± 9.22</td></tr><tr><td class="align_left">Processed stability (autosampler, 48 h)</td><td class="align_center">107.78 ± 11.05</td><td class="align_center">101.54 ± 3.42</td></tr><tr><td class="align_left">Processed stability (4°C, 48 h)</td><td class="align_center">100.61 ± 4.02</td><td class="align_center">97.39 ± 6.07</td></tr><tr><td class="align_left">Benchtop stability (2 IU/mL ASNase, 48 h)</td><td class="align_center">98.56 ± 0.54</td><td class="align_center">93.76 ± 3.56</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Stability of ASN in precipitated human serum samples under different storage conditions (<i>n</i> = 3).</div>

International Journal of Analytical Chemistry

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Table 3

Table 3: Development and Validation of a Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry Method for the Determination of Asparagine in Human Serum 

Table 3 | Development and Validation of a Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry Method for the Determination of Asparagine in Human Serum 