An UPLC-MS/MS Method for Determination of Osimertinib in Rat Plasma: Application to Investigating the Effect of Ginsenoside Rg3 on the Pharmacokinetics of Osimertinib

Table 5

Stability of osimertinib in rat plasma under various storage conditions (n = 6).

Spiked concentration (ng/mL)	Short-term (room temperature for 6 h)			Autosampler (4°C for 24 h)			Freeze-thawing (−80°C for 3 cycles)			Long-term (−80°C for 28 days)
	Mean ± SD (ng/mL)	RSD (%)	RE (%)	Mean ± SD (ng/mL)	RSD (%)	RE (%)	Mean ± SD (ng/mL)	RSD (%)	RE (%)	Mean ± SD (ng/mL)	RSD (%)	RE (%)
3	3.20 ± 0.14	4.2	6.8	3.02 ± 0.01	0.4	0.6	3.03 ± 0.02	0.8	1.0	2.96 ± 0.24	8.2	−1.5
30	30.1 ± 1.0	3.1	0.4	28.7 ± 0.8	2.7	−4.2	30.1 ± 1.0	3.1	0.4	32.7 ± 0.8	2.3	9.1
350	341 ± 3	0.8	−2.7	359 ± 26	7.1	2.5	349 ± 6	1.6	−0.3	359 ± 7	2.0	2.4