International Journal of Analytical Chemistry

Research Article

Systematic Analysis of the Whole-Body Tissue Distribution and Fatty Acid Compositions of Membrane Lipids in CD1 and NMRI Mice and Wistar Rats

Table 1

Quantitation of 11 lipid classes in 12 organs/tissues from CD1 mice, NMRI mice, and Wistar rats.

Animal species

CD1 mice

NMRI mice

Rat

CD1 mice

NMRI mice

Rat

CD1 mice

NMRI mice

Rat

CD1 mice

NMRI mice

Rat

(a) Includes the data from brain, fat, heart, and kidney

Organs

Brain

Fat

Heart

Kidney

470.0 ± 63.0

197.8 ± 36.5

168.4 ± 10.9

8.4 ± 3.6

11.5 ± 7.4

3.2 ± 1.2

252.6 ± 19.8

220.7 ± 17.8

381.9 ± 32.5

458.0 ± 33.6

257.5 ± 9.2

444.6 ± 35.7

LPC

16.1 ± 2.3

17.0 ± 3.4

35.7 ± 5.0

2.1 ± 1.3

13.5 ± 9.0

2.5 ± 1.4

93.2 ± 12.2

115.5 ± 23.6

60.6 ± 22.8

55.2 ± 8.5

66.2 ± 6.2

31.2 ± 1.7

LPE

8.0 ± 1.9

20.4 ± 4.3

8.9 ± 1.6

0.8 ± 0.7

7.9 ± 8.1

n.d. ± n.d.

43.2 ± 4.8

57.0 ± 12.9

44.2 ± 22.0

48.0 ± 5.2

50.0 ± 7.7

24.8 ± 3.1

LPS

n.d. ± n.d.

0.1 ± 0.0

n.d. ± n.d.

4079.0 ± 218.9

2908.9 ± 375.9

4912.5 ± 442.5

210.7 ± 36.2

436.5 ± 139.1

426.4 ± 66.6

2469.4 ± 125.0

2368.0 ± 121.1

4007.6 ± 244.6

3322.6 ± 245.9

3192.4 ± 68.4

2966.7 ± 169.7

5120.3 ± 267.5

6704.8 ± 1142.4

13374.3 ± 556.7

189.2 ± 53.6

433.1 ± 135.7

246.2 ± 44.9

1635.3 ± 118.7

1638.6 ± 114.9

3613.5 ± 238.6

3377.3 ± 156.7

2500.0 ± 152.1

5495.7 ± 333.7

95.0 ± 4.7

73.6 ± 6.8

50.4 ± 3.8

0.2 ± 0.2

3.4 ± 2.0

0.2 ± 0.1

61.8 ± 6.9

44.1 ± 5.4

49.2 ± 5.7

161.7 ± 32.1

73.9 ± 8.6

30.7 ± 2.2

294.2 ± 22.9

235.5 ± 28.2

194.0 ± 10.1

16.0 ± 5.6

19.1 ± 8.2

8.7 ± 3.3

89.9 ± 8.1

45.0 ± 5.6

35.5 ± 4.6

405.8 ± 47.1

126.8 ± 9.6

167.4 ± 7.8

1244.6 ± 41.4

1179.6 ± 87.7

1831.2 ± 137.0

59.3 ± 12.1

68.0 ± 12.8

24.5 ± 8.1

78.0 ± 7.4

44.8 ± 9.0

71.0 ± 15.0

462.9 ± 33.9

333.6 ± 38.2

606.9 ± 21.0

20.7 ± 5.5

13.0 ± 4.0

155.1 ± 61.7

133.8 ± 23.5

92.9 ± 10.0

262.6 ± 36.0

9.9 ± 5.4

3.6 ± 1.1

47.3 ± 8.6

14.6 ± 2.7

8.1 ± 0.9

42.6 ± 6.8

3.4 ± 1.5

26.9 ± 23.3

28.0 ± 10.2

5073.4 ± 879.5

3093.1 ± 308.5

19115.4 ± 3094.0

292.7 ± 99.0

110.8 ± 82.7

490.0 ± 248.2

231.7 ± 92.0

167.4 ± 53.0

298.8 ± 73.4

Acidic phospholipids

2103.8

1686.6

2244.1

83.9

101.9

36.6

482.4

354.6

537.7

1488.4

791.7

1249.6

Neutral phospholipids

9223.4

9651.1

18331.3

402.7

891.0

675.1

4241.1

4179.1

7725.9

6803.1

5808.5

8518.4

Neutral lipids

24.1

39.9

183.1

5207.2

3186.1

19378.0

302.6

114.4

537.3

246.3

175.5

341.4

(b) Includes the data from L-intestine, liver, lung, and muscle

Organs

L-intestine

Liver

Lung

Muscle

97.2 ± 18.0

77.2 ± 10.1

64.2 ± 9.2

164.1 ± 25.3

151.6 ± 9.8

282.4 ± 39.2

35.8 ± 3.5

10.4 ± 1.6

28.7 ± 9.6

79.8 ± 6.4

55.5 ± 6.6

123.7 ± 17.6

LPC

73.7 ± 12.0

113.6 ± 36.5

101.8 ± 20.0

111.5 ± 25.2

192.7 ± 94.5

102.7 ± 39.4

15.9 ± 3.1

28.2 ± 6.2

7.9 ± 2.0

53.8 ± 14.6

22.8 ± 6.4

87.9 ± 36.4

LPE

47.8 ± 10.2

103.4 ± 27.1

17.2 ± 7.9

27.8 ± 9.8

98.8 ± 54.9

11.8 ± 7.3

10.7 ± 0.9

24.1 ± 6.6

n.d. ± n.d.

14.8 ± 5.6

6.6 ± 2.3

21.1 ± 13.8

LPS

n.d. ± n.d.

0.2 ± 0.0

n.d. ± n.d.

0.1 ± 0.0

n.d. ± n.d.

1728.9 ± 196.6

2329.0 ± 268.0

1977.2 ± 157.7

3209.5 ± 183.6

3795.9 ± 902.6

6245.5 ± 438.0

517.8 ± 40.8

855.0 ± 78.5

624.0 ± 191.7

1871.5 ± 151.3

1287.8 ± 84.9

6463.1 ± 382.7

1546.7 ± 177.8

2882.2 ± 370.4

2463.5 ± 333.4

2260.5 ± 118.6

1942.5 ± 449.9

4535.3 ± 270.0

1511.3 ± 55.8

1647.3 ± 238.2

2658.6 ± 687.5

1862.0 ± 169.6

1471.3 ± 135.5

3341.1 ± 164.8

38.8 ± 7.1

29.3 ± 3.6

15.9 ± 2.1

30.9 ± 9.6

47.7 ± 2.4

20.2 ± 2.6

449.6 ± 48.5

216.2 ± 28.4

165.4 ± 72.8

23.4 ± 4.0

15.0 ± 2.6

12.3 ± 1.3

146.5 ± 22.0

120.9 ± 12.4

74.3 ± 12.3

104.6 ± 25.2

129.5 ± 7.8

166.1 ± 26.3

157.9 ± 8.1

43.1 ± 7.0

41.9 ± 18.0

47.3 ± 8.1

35.3 ± 7.1

30.6 ± 4.2

278.3 ± 29.3

323.9 ± 43.1

138.2 ± 11.6

203.3 ± 41.1

124.6 ± 23.7

260.7 ± 113.0

555.9 ± 28.3

171.6 ± 25.4

381.8 ± 174.6

70.9 ± 8.1

65.0 ± 9.5

54.3 ± 5.9

29.7 ± 5.1

24.9 ± 7.9

51.6 ± 9.5

57.8 ± 11.2

57.2 ± 18.4

101.2 ± 20.6

25.3 ± 3.4

16.7 ± 2.2

71.6 ± 19.3

8.3 ± 2.3

11.6 ± 3.6

67.8 ± 40.6

1084.7 ± 288.1

1286.6 ± 475.6

2423.6 ± 1000.5

392.4 ± 96.9

734.6 ± 161.0

602.5 ± 207.8

323.1 ± 54.3

279.5 ± 92.0

164.5 ± 66.7

1894.2 ± 258.9

815.7 ± 277.9

2110.8 ± 1380.0

Acidic phospholipids

560.8

551.4

292.8

502.9

453.4

729.3

1199.2

441.5

617.8

221.4

170.9

220.8

Neutral phospholipids

3397.1

5428.2

4559.7

5609.4

6030.0

10895.4

2055.8

2554.6

3290.6

3802.1

2788.5

9913.2

Neutral lipids

1114.4

1311.4

2475.2

450.2

791.8

703.7

348.4

296.2

236.1

1902.5

827.3

2178.6

Organs

S-intestine

Skin

Spleen

Stomach

91.5 ± 17.9

23.1 ± 9.7

60.9 ± 21.0

20.3 ± 4.0

13.2 ± 1.7

18.3 ± 3.8

57.7 ± 5.9

29.7 ± 1.7

35.3 ± 2.5

44.0 ± 21.8

6.0 ± 2.6

12.7 ± 3.4

LPC

47.7 ± 14.3

71.5 ± 38.3

87.5 ± 34.9

9.4 ± 1.6

13.4 ± 2.1

7.1 ± 0.9

27.7 ± 6.5

27.5 ± 3.9

24.8 ± 3.0

329.7 ± 150.5

256.6 ± 86.6

218.0 ± 35.2

LPE

51.1 ± 25.4

111.9 ± 30.4

45.5 ± 21.2

1.9 ± 0.9

3.4 ± 0.6

1.0 ± 0.7

10.6 ± 2.0

18.2 ± 2.6

3.4 ± 0.9

272.3 ± 105.9

145.1 ± 56.9

24.0 ± 2.8

LPS

n.d. ± n.d.

0.1 ± 0.0

n.d. ± n.d.

225.8 ± 66.0

23.5 ± 6.8

5.5 ± 2.1

1754.5 ± 222.9

937.6 ± 350.3

2402.0 ± 1130.7

413.8 ± 82.5

691.4 ± 45.5

1075.8 ± 109.5

2084.8 ± 58.3

1613.7 ± 58.8

1670.9 ± 121.3

1642.8 ± 309.9

812.5 ± 158.7

879.8 ± 45.9

1419.6 ± 184.6

589.4 ± 172.2

1865.2 ± 388.3

300.5 ± 30.7

549.4 ± 29.0

630.8 ± 72.0

1356.9 ± 60.7

1238.4 ± 74.1

1769.8 ± 50.5

1122.0 ± 235.3

582.6 ± 118.9

1203.4 ± 138.8

70.9 ± 18.1

25.1 ± 13.1

7.8 ± 3.4

10.5 ± 2.14

6.2 ± 0.8

2.5 ± 0.4

37.1 ± 2.8

35.9 ± 2.7

29.9 ± 4.1

22.3 ± 4.8

16.0 ± 4.1

3.6 ± 0.9

165.9 ± 29.9

52.1 ± 20.1

27.0 ± 11.7

44.2 ± 5.5

30.0 ± 2.2

16.1 ± 2.7

83.0 ± 11.3

52.8 ± 6.5

41.4 ± 3.8

87.2 ± 16.0

55.3 ± 8.9

14.9 ± 4.9

231.0 ± 56.5

74.6 ± 15.6

106.0 ± 31.1

98.9 ± 12.

89.1 ± 6.8

17.3 ± 3.7

298.6 ± 25.2

195.1 ± 25.8

246.1 ± 40.9

84.9 ± 22.3

47.4 ± 10.8

37.0 ± 9.0

30.8 ± 6.5

13.6 ± 3.4

235.3 ± 87.3

12.9 ± 3.2

17.5 ± 2.2

60.7 ± 17.3

5.7 ± 1.5

4.0 ± 0.6

9.7 ± 0.9

25.3 ± 6.7

11.6 ± 3.3

27.7 ± 4.9

616.1 ± 409.5

343.9 ± 233.2

964.7 ± 544.7

1316.8 ± 230.5

1270.5 ± 192.6

4080.5 ± 1091.7

127.7 ± 76.1

37.8 ± 20.0

133.1 ± 101.7

589.4 ± 288.4

328.2 ± 183.3

1365.5 ± 395.1

Acidic phospholipids

559.4

174.9

201.7

173.9

138.6

54.3

476.4

313.5

352.7

464.2

148.1

73.7

Neutral phospholipids

3272.9

1710.4

4400.2

725.6

1257.6

1714.7

3480.1

2897.8

3468.9

3366.8

1796.9

2325.3

Neutral lipids

647.0

357.5

1199.9

1329.8

1288.0

4141.3

133.4

41.8

142.7

614.7

339.7

1393.3

The unit of all the measurements is ng/mg organ/tissue. Nine replicates were used for measurement.