Determination of Crizotinib in Mouse Tissues by LC-MS/MS and Its Application to a Tissue Distribution Study

Table 1

The differences between the current method and some previously developed methods.

Sample	Linear range (ng mL)	Retention time (min)	Column	Sample preparation method	Mobile phase	Reference
Mouse tissue	20–8000	1.31	Acquity UPLC HSS T3 column (1.8 μm, 100 mm × 2.1 mm i.d.)	Protein precipitation	Methanol (solvent A) and 0.3% formic acid water solution (solvent B)	Current method
Human plasma	50–5000	4.40	Gemini C18 column (5.0 μm, 50 × 2.0 mm i.d.)	Protein precipitation.	10 mM ammonium bicarbonate in water and 10 mM ammonium bicarbonate in methanol-water (1 : 9, v/v)	[11]
Human plasma	4–800	1.17	CORTECS® C18 UPLC column (dp = 1.6 μm, 2.1 × 50 mm)	Solid-phase extraction	0.01% acetic acid buffer in water (solvent A) and acetonitrile added with 10% A	[12]
Human plasma	5–1000	3.80	Accucore® C18 column (2.1 × 50 mm, 2.6 μm)	Protein precipitation	0.1% (v/v) formic acid and acetonitrile containing 0.1% (v/v) formic acid	[13]
Human plasma	0.1–1000	1.22	ACQUITY UPLC BEH C18 column (50 × 2.1 mm, i.d., 1.7 μm)	Protein precipitation and dried under nitrogen gas	0.1% formic acid aqueous and acetonitrile/methanol (v : v, 1 : 1)	[15]
Rat plasma	1–2000	1.65	Agilent zorbax XDB C18 column (2.1 × 50 mm, 3.5 μm)	Protein precipitation	0.1% formic acid and methanol containing 0.1% formic acid	[16]
Mouse plasma	10–10000	1.2	Acquity UPLC^® BEH C18 column (30 mm × 2.1 mm, dp = 1.7 μm)	Protein precipitation	0.1% (v/v) ammonium hydroxide and methanol	[17]