Determination of Crizotinib in Mouse Tissues by LC-MS/MS and Its Application to a Tissue Distribution Study

Table 3

Precision and accuracy data for the quantification of crizotinib in mouse tissue homogenates (n = 5).

Tissues	Concentration levels (mean ± SD, ng/mL)		RSD (%)		RE (%)
	Added	Measured	Intraday	Interday	Accuracy
Liver	20	19.45 ± 2.06	10.9	8.6	−2.7
	60	62.80 ± 4.70	5.4	14.8	4.7
	600	602.2 ± 60.31	10.5	6	0.4
	6400	5875 ± 384	3.6	14.8	−8.2
Small intestine	20	19.92 ± 1.51	8.2	1.7	−0.4
	60	58.95 ± 4.16	7.3	5.8	−1.8
	600	554.6 ± 32.47	6.3	1.2	−7.6
	6400	5739 ± 256	4.7	2.2	−10.3

RSD, relative standard deviation; RE, relative error; n, number of replicates.