A Validated Method for the Simultaneous Determination of Methamphetamine and 3,4-Methylenedioxy-N-methamphetamine in Blood-Based Liquid Chromatography-Tandem Mass Spectrometry

Table 1

Operating conditions of HPLC-MS/MS for determination MA and MDMA in blood.

Instrument parameter
HPLC-Agilent 1290 Infinity
Autosampler	Agilent G4226 A
Column	Agilent eclipse plus C18 RRHD analytical column, 2.1 × 50 mm, 1.8 µm (959757–902)
Column temperature	30°C
Flow rate	0.3 mL/min
Mobile phase	Water contains 0.1% formic acid (A) and acetonitrile (B)
Gradient solvent (%B, min)	Initial, 80%; 1 min, 80%; 2.2 min, 100%; 2.8 min, 80%
Injection volume	5 µL
MS-Agilent 6490 Triple Quad
Capillary (vcap)	4000 V (positive)
Gas temperature	350°C
Gas flow	He, 11 L/min
Nebulizer	35 psi
Nozzle voltage	1500 V (positive)
RF	110–200 V (positive)