A Validated Liquid Chromatographic Method for Berberine Analysis in Tissue and Application

Table 1

The calibration curves, LOD, and LOQ of BB in different tissues (n = 3).

Samples	Regression equation	Correlation coefficients (r)	Linear range (μg/g)	LOD (μg/g)	LOQ (μg/g)
Liver	y = 36033x − 664	0.9999	0.025–10.0	0.025	0.040
Kidney	y = 34212x − 380	0.9996	0.020–0.50	0.017	0.036
Spleen	y = 31092x − 555	0.9993	0.025–0.50	0.023	0.035