A Validated Liquid Chromatographic Method for Berberine Analysis in Tissue and Application

Table 3

Stability of BB in samples (n = 3).

Tissues	Concentration (μg/g)	Freeze-thaw cycle RSD (%)	Prepreparative RSD (%)	Postpreparative RSD (%)	−20°C for 2 weeks RSD %
Liver	0.25	4.2	7.5	3.9	9.2
	1.25	3.8	6.4	4.6	9.5
	10.0	3.5	7.1	4.2	9.1
Kidney	0.10	2.1	2.7	1.3	1.1
	0.25	2.4	4.3	2.9	1.9
Spleen	0.10	3.8	4.2	3.3	3.7
	0.25	2.1	2.1	1.6	4.4