Prevalidation of an ELISA for Detection of a New Clinical Entity: Leishmania donovani-Induced Cutaneous Leishmaniasis

Figure 1

Schematic representation of indirect ELISA method used for the study. (i) Crude Leishmania Ag, (ii) anti-Leishmania IgG Ab in human serum, (iii) peroxidase-goat anti-human IgG secondary Ab, (iv) HRP, (v) TMB substrate, and (vi) oxidized TMB.