Derivatization of Levoglucosan for Compound-Specific δ¹³C Analysis by Gas Chromatography/Combustion/Isotope Ratio Mass Spectrometry

Table 2

Measured and predicted stable carbon isotopic compositions of levoglucosan and LMT.

Suppliers	δ^13C^a
	Measured levoglucosan^b,c	Measured LMT^b,d,e	Calculated LMT ^f	Δ^g
S1	−15.65 ± 0.08; (n = 3)	−24.53 ± 0.13; (n = 3)	−24.32	0.21
S2	−14.45 ± 0.03; (n = 3)	−23.69 ± 0.21; (n = 3)	−23.60	0.09
S3	−12.20 ± 0.10; (n = 3)	−22.61 ± 0.18; (n = 4)	−22.25	0.36
S4	−27.48 ± 0.07; (n = 3)	−31.56 ± 0.18; (n = 4)	−31.42	0.14

^aStable carbon isotopic compositions reported in per mil relative to PDB; ^bthe arithmetic means and standard deviations; ^cδ¹³C values determined by EA/IRMS; ^dδ¹³C values determined by GC/C/IRMS; ^eδ¹³C_MBA = −59.42 ± 0.13‰ (n = 3, determined by EA/IRMS) δ¹³C_TMS = −29.96 and was obtained by reacting the same batch of MSTFA with myo-inositol standard (δ¹³C = −14.65 ± 0.03‰; n = 3, determined by EA/IRMS); ^fon the basis of the mass balance relationship equation (1); ^gcalculated δ¹³C_LMT-measured δ¹³C_LMT; n: number of measured times.