Derivatization of Levoglucosan for Compound-Specific δ¹³C Analysis by Gas Chromatography/Combustion/Isotope Ratio Mass Spectrometry

Table 3

δ¹³C values of microcrystalline cellulose and its combustion particle product levoglucosan under four temperatures.

Combustion temperature (°C)	δ^13C (‰)
	Measured cellulose by EA/RMS	Measured LMT by GC/C/IRMS	Calculated levoglucosan^a	Δ^b
300	−25.15	−27.27 ± 0.31	−23.24	−1.91
400		−27.34 ± 0.22	−23.42	−1.73
500		−27.55 ± 0.15	−23.93	−1.21
600		−27.40 ± 0.19	−23.56	−1.59

^aOn the basis of equation δ¹³C_levoglucosan = f_LMTδ¹³C_LMT − f_MBAδ¹³C_MBA − f_TMSδ¹³C_TMS, f_LMT = 5/3, f_MBA = 1/6, f_TMS = 1/2, δ¹³C_MBA = −59.42‰, and δ¹³C_TMS = −29.96‰; ^bmeasured δ¹³C_cellulose − calculated δ¹³C_levoglucosan.