Simultaneous Determination of Amphenicols and Metabolites in Animal-Derived Foods Using Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry

Table 6

Recovery and precision of amphenicols and metabolites (n = 6).

Matrix	Analyte	Added level (μg/kg)	Recovery (%)	Intraday RSD (%)	Interday RSD (%)
Pork	CAP	1	98.93	4.90	6.38
		5	115.81	2.16	3.71
		10	113.16	5.00	5.29
	TAP	1	95.80	8.30	9.11
		5	112.92	7.44	7.82
		10	106.31	6.93	7.92
	FF	1	83.81	5.62	5.68
		5	93.02	2.82	4.59
		10	95.63	1.83	4.12
	FFA	5	97.07	2.51	6.24
		10	89.22	6.23	10.42
		50	89.61	7.93	11.94
Beef	CAP	1	113.00	7.44	7.31
		5	99.73	4.41	8.24
		10	93.30	2.50	10.35
	TAP	1	87.91	5.53	9.47
		5	93.99	5.10	11.90
		10	80.10	3.02	7.10
	FF	1	80.13	6.08	8.22
		5	89.50	4.90	4.93
		10	96.81	2.12	2.41
	FFA	5	86.43	9.22	9.60
		10	80.29	7.18	7.22
		50	100.22	3.90	10.8
Lamb	CAP	1	97.20	1.83	2.30
		5	110.91	2.61	6.00
		10	120.30	3.88	7.43
	TAP	1	71.77	6.15	6.20
		5	105.44	4.50	4.86
		10	99.02	1.93	2.83
	FF	1	81.21	6.27	12.30
		5	109.00	1.21	1.37
		10	99.03	1.90	4.19
	FFA	5	101.14	4.58	11.00
		10	103.91	5.24	8.25
		50	92.02	3.72	8.33
Chicken	CAP	1	101.27	4.71	8.34
		5	101.05	1.63	3.60
		10	97.03	5.74	5.42
	TAP	1	84.00	6.88	11.70
		5	105.23	6.30	8.70
		10	103.54	6.91	11.02
	FF	1	101.10	3.90	5.22
		5	85.44	7.16	9.70
		10	82.82	4.28	4.21
	FFA	5	108.71	6.30	5.54
		10	101.63	6.62	11.30
		50	89.90	7.30	7.64

CAP, chloramphenicol; FF, florfenicol; FFA, florfenicol amine; RSD, relative standard deviation; TAP, thiamphenicol.