Simultaneous and Rapid Determination of Six Tyrosine Kinase Inhibitors in Patients with Non-Small Cell Lung Cancer Using HPLC-MS/MS

Table 1

Optimized mass spectrometry parameters of six analytes and IS.

Analytes	Ionization mode (±)	Precursor ions (m/z)	Fragmentor (V)	Collision energy (V)	Product ions (m/z)
Gefitinib	+	447.2	90	22	128.2
Icotinib	+	392.1	155	32	304.2
Afatinib	+	486.2	140	32	371.1
Erlotinib	+	394.2	100	30	278.1
Crizotinib	+	450.2	140	26	260.1
Osimertinib	+	499.9	105	26	72.0
Carbamazepine (IS)	+	237.1	115	19	194.1