A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT₃ Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker

Table 5

Effects of instrument, column, flow rate, pH value of mobile phase, and column temperature upon RRT of the five 5-HT₃ receptor antagonists.

Compound	RRT	RSD (%)
		Instrument	Column	Flow rate	Temperature	pH value
Azasetron	0.431	0.3	4.2	0.9	0.5	2.3
Granisetron	0.679	0.7	2.8	1.3	0.9	1.9
Tropisetron	0.812	0.3	2.1	0.4	1.2	2.5
Ondansetron	1.000	0	0	0	0	0
Ramosetron	1.132	0.5	2.6	0.6	0.6	1.6